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Speeding the use of centrifuges Notes |
Author: Date:2009-11-28 |
First,centrifuges steps
1. Connected to power, open the cover of centrifuges.
2. Requested by the assembly of centrifuges tube well.
3. Centrifugal required to install the other way round.
4. Closed to build centrifuges.
5. Centrifugal input data for centrifuges program.
6. Vacuum, and began to run the program.
7. After the procedure, to a vacuum.
8. centrifuges open the cover, take out the other way round.
9. centrifuges tubes removed and analyzed.
Second, the selection of centrifuges tube
1. Centrifugal glass tube can not in the high speed centrifuges use.
2.PA control: chemical properties of stable, semi-transparent, high temperature can be disinfected.
3.PP control: chemical properties of stable, semi-transparent, high temperature can be disinfected. PC control: transparency, hardness, high temperature can be disinfected. However, intolerance strong acid and strong base of some organic solvents. For 50,000 (transfer / min) than centrifugal. CN control: soft texture, transparent, but the impatience of some strong acid and strong base of organic solvents, high pressure can not be disinfected. Suitable for sugar, such as glycerol density gradient centrifugation. Should be transparent and conducive to the collection.
Symmetrical balance: When the centrifugal speed up to 1-5 million (transfer / min), such as the symmetric difference of 1 gram tube, turned a radius of 5 cm, the centrifugal force formula F = m × RCF
A look-up table: 10,000 (transfer /) RCF = 6000 into the formula F = 1 × 6000 = 6 (kg)
50,000 (transfer / min) RCF = 150000 into the formula F = 1 × 150000 = 150 (kg)
At this point rise up on both sides of the imbalance 6-150 (kg), which is the centrifuges is a lot of damage, at least to shorten the useful life of centrifuges.
4, such as poor sealing lid centrifuge tubes of liquid will not be able to fill (for high-speed centrifuges and the first point of use) in order to prevent spillover.
Spillover consequences: pollution and turn their back on the centrifugal chamber, the impact sensor to work
5, ultracentrifugation, the liquid must fill tube should be from the ultra-vacuum when only fill tube in order to avoid distortion.
6, when the first point of use, do not forget to cover her head covered, if built, will have a great centrifugal cavity of the eddy current friction and heat resistance, which is equivalent to the centrifuges motor and an additional burden on the refrigerator, the impact of centrifuges Life.
Third, the maintenance Notes
1.symmetrical balance: When the centrifugal speed up to 1-5 million (transfer / min), such as the symmetric difference of 1 gram tube, turned a radius of 5 cm, the centrifugal force formula F = m × RCF
A look-up table: 10,000 (transfer /) RCF = 6000 into the formula F = 1 × 6000 = 6 (kg)
50,000 (transfer / min) RCF = 150000 into the formula F = 1 × 150000 = 150 (kg)
At this point rise up on both sides of the imbalance 6-150 (kg), which is the centrifuges is a lot of damage, at least to shorten the useful life of centrifuges.
2.centrifuge tubes, such as poor fluid sealing lid will not be able to fill (for high-speed centrifuges and the first point of use) in order to prevent spillover. Spillover effects of pollution and turn their back on the centrifugal chamber and loss of balance, the impact sensor to work
3.ultracentrifugation, the liquid must fill tube should be from the ultra-vacuum when only fill tube in order to avoid distortion.
4, for the first time the use of point of view, do not forget to cover her head covered, if built, will have a great centrifugal cavity of the eddy current friction and heat resistance, which is equivalent to the centrifuges motor and an additional burden on the refrigerator, the impact of centrifuges Life.
Fourth, attention to maintenance issues
1. centrifuges cavity clean and turned and wiped away condensation water cavity.
2. After using the fight to open the door until the cavity to restore normal temperature.
3. centrifuges to balance the samples before.
4. centrifuges and then they have to pay attention to disinfection and sterilization.
5. Based on the need to use tubes / bottles, adapters and spacers, in accordance with the requirements to increase the volume of samples.
6. Rotor pre-cooling, if necessary.
7. Turn their back on maintenance and storage, the need to protect the ring at the bottom of the count.
8. Accurate tools to use and turn their back on the correct installation. |