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The type of centrifuges and centrifuge used to introduce laboratory |
| Author： Date：2008-10-10 |
1. Type:
laboratory use and industrial use, intermittent and continuous, frozen and non-frozen-ground-with-tables, general and high-speed centrifuges and centrifuge speed centrifuge, preparation and analysis-type.
2. Laboratory centrifuges used:
General centrifuges - desktop (3000-16000rpm) and ground-(3000-6000rpm), generally without refrigeration equipment, electrical and from the rotor, by the speed regulation of voltage regulator.
High-speed refrigerated centrifuge - a desktop with the ground-,18000-25000rpm ,0-4.
+ Above refrigeration equipment and temperature detectors.
Speed centrifuges - the ground-up 30000rpm ,0-4.
+ Above vacuum equipment and vacuum detector.
3. Rotor: Kok-and rotor-level, angle-type centrifuge used in the settlement, also used in the horizontal gradient centrifugation.
4. Centrifugal principle: the use of high-speed rotating rotor when the power generated by the centrifugal force, to accelerate the speed of precipitation particles, the samples of different factors or settlement buoyant density material separated.
F = 2r. is the angular velocity (radians / sec). r is the radius of the particle's spin (cm)
F is any particle withstand the outward centrifugal force.
RCF = 2r/980, RCF is the relative centrifugal force, the unit for the G; 980 is gravity, the unit cm / s ?.
RCF = 119 / 10-5n2r, n for the revolution per minute.
In a centrifuge tube in different positions of the particles suffered centrifugal force, often rotating centrifuge tube to the mid-point of the Center for the distance r when the relative centrifugal plans have been expressed as an average of centrifugal force.
The centrifugal force of different calculation speed and centrifugal radius of the centrifuge, into the same centrifuge to measure particles in the centrifugal suffered in the size of the force applied to different as a result of the use of centrifuges, the rotor in a different choice in the use of centrifugal time And speed.
Centrifugal time: t = [( r2- r1) / 2] / s
t for the full sample particles to the settlement of the centrifuge tube at the end of time (seconds).
S factor for the settlement.
r2 to the center axis of rotation for the centrifuge tube at the bottom of the distance (cm).
r1 to the center axis of rotation for the sample of liquid from the surface (cm). |